《植物生理学报》 2015, 51(3): 354-362

樱桃MADS-box转录因子的生物信息学及其表达分析

朱友银^1,2,*, 邵姁^2,*, 王月², 李永强², 郭卫东^2,**

¹金华职业技术学院农业与生物工程学院, 浙江金华321007; ²浙江师范大学浙江省特色经济植物生物技术研究重点实验室, 浙江金华321004

通信作者：朱友银；E-mail: gwd@zjnu.cn；Tel: 0579-82282641

摘　要：

MADS-box转录因子在植物花和果实发育过程中发挥重要调控作用。本文从樱桃花芽转录组分析中, 获得18个具有全长开放阅读框的MADS-box转录因子(PpcMADS), 利用生物信息学方法对它们编码的氨基酸基本性质和结构进行了系统分析。结果表明, 除KP347550和KP347552以外, 均属于MIKC家族, 大部分为不稳定碱性亲水蛋白, 二级结构中同时含有α-螺旋、β-转角、扩展链和无规则卷曲结构, 其中α-螺旋所占比例最高。亚细胞定位分析显示, MADS-box蛋白主要定位在细胞核中, 而KP347549定位到细胞质、KP347545和KP347552定位到质膜的几率较高。序列主要含有4个保守基序, 其中基序1为该基因家族的保守基序, 含有MADS盒。PpcMADS转录因子可以分为AP3亚组、PI亚组、SHP亚组、AG亚组、AP1亚组、SEP亚组、SOC亚组、SVP亚组及4个未知亚组。KP347545、KP347546、KP347547、KP347549、KP347551、KP347552、KM243373、KM243375和KM243377只在花中表达, KP347548和KP347550在花和果实中均不表达, 其他7个基因在两个组织中都表达。

关键词：樱桃; MADS-box; 转录因子; 生物信息分析; 表达分析

收稿：2015-01-20   修定：2015-02-14

资助：浙江省自然科学基金(LQ13C150001)和浙江省新品种选育重大专项(2012C12904-6)。

Bioinformatics Analysis of MADS-box Genes and Their Expression in Cherry

ZHU You-Yin^{1, 2,*}, SHAO Xu^2,*, WANG Yue², LI Yong-Qiang², GUO Wei-Dong^2,**

¹College of Agricultural and Biological Engineering, Jinhua Polytechnic, Jinhua, Zhejiang 321007, China; ²Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, Zhejiang Normal University, Jinhua, Zhejiang 321004, China

Corresponding author: ZHU You-Yin; E-mail: gwd@zjnu.cn; Tel: 0579-82282641

Abstract:

MADS-box gene family is a large group which plays important roles in regulation of plant growth and development. Totally, 18 MADS-box genes (PpcMADS) with full ORF were identified from transcriptome generated from flower bud of cherry. In order to reveal the function of PpcMADS, the property and structure of proteins were analyzed using bioinformatics methods. The results showed that 16 PpcMADS genes belonged to MIKC family, while KP347550 and KP347552 were the members of M-type. Most of them were unstable hydrophilic proteins. The secondary structure of PpcMADS protein included alpha helix, beta turn, extended strand and random coil, and alpha helix was major part. Most of PpcMADS proteins located in nucleus, while KP347549 may locate in cytoplasm, KP347545 and KP347552 were more likely to locate in plasma membrane. Four motifs were identified from PpcMADS protein, and the Motif 1 was the conserved structure of MADSbox family contained MADS domain. The result of phylogenetic analysis revealed that 18 PpcMADS genes could be divided into AP3 subclass, PI subclass, SHP subclass, AG subclass, AP1 subclass, SEP subclass, SOC subclass, SVP subclass and four unknown subclass. KP347545, KP347546, KP347547, KP347549, KP347551, KP347552, KM243373, KM243375 and KM243377 only expressed in flower, KP347548 and KP347550 did not expressed in flower and fruit, while the other 7 PpcMADS genes expressed in fruit and flower.

Key words: cherry; MADS-box; transcription factor; bioinformatics; expression analysis